alpha view sa 3.4.0 software Search Results


horseradish peroxidase hrp  (Dojindo Labs)
95
Dojindo Labs horseradish peroxidase hrp
Horseradish Peroxidase Hrp, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d-glucose hk, uv method test kit  (NZYTech Inc)
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NZYTech Inc d-glucose hk, uv method test kit
D Glucose Hk, Uv Method Test Kit, supplied by NZYTech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glutathione reductase (gsh) assay kit bioxytech gr-340  (OXIS International)
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OXIS International glutathione reductase (gsh) assay kit bioxytech gr-340
Glutathione Reductase (Gsh) Assay Kit Bioxytech Gr 340, supplied by OXIS International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gpx-340 assay kit  (OXIS International)
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OXIS International gpx-340 assay kit
Gpx 340 Assay Kit, supplied by OXIS International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bioxytech aconitase-340 kit  (OXIS International)
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OXIS International bioxytech aconitase-340 kit
Bioxytech Aconitase 340 Kit, supplied by OXIS International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tubulin polymerization  (Molecular Devices LLC)
96
Molecular Devices LLC tubulin polymerization
Diclofenac induces microtubule depolymerization. ( A ) Confocal microscopy of HeLa cells stained with antibodies to <t>α-tubulin</t> (green) after incubation at 37 °C (upper panel) for 6 h or at 37 °C for 3 h followed by 4 °C for 3 h. Cells were incubated in a medium containing vehicle (0.1% dimethyl sulfoxide [DMSO]), nocodazole (100 nM), taxol (100 nM), or diclofenac (170 µM, 200 µM). Nuclei were stained with 4–6-diamidino-2-phenylindole (DAPI, blue). ( B ) Quantitative analysis of mean fluorescence intensity of α-tubulin. Data are presented as means ± SD from three independent experiments ( n = 52–60 cells). * p < 0.05; ** p < 0.01 (Student’s t -test). ( C ) Confocal microscopy of HepG2 cells stained with antibodies to α-tubulin (green) and LAMP1 (red) after incubation in a medium containing vehicle (0.1% DMSO) or diclofenac (500 µM) under fed (Dulbecco’s modified eagle medium, 10% fetal bovine serum) conditions or starved (Earle’s balanced salt solution) conditions for 8 h. Nuclei were stained with DAPI (blue). Representative images are shown. ( n = 14–18 cell). Areas enclosed by the white boxes are shown at higher magnification. Yellow boxes indicate the edges of the plasma membrane. Three independent experiments were performed. Scale bar, 10 µm; scale bar in magnification; 2 µm. ( D ) In vitro <t>tubulin</t> <t>polymerization.</t> Polymerization activity was monitored in the presence of DMSO (0.01%, vehicle), taxol (10 μM), nocodazole (10 μM), or diclofenac (0.17 mM and 1.7 mM) for 30 min at 37 °C as the increase in A 340 nm. Two independent experiments were performed.
Tubulin Polymerization, supplied by Molecular Devices LLC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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il-3 cytokine  (Immunex Corporation)
90
Immunex Corporation il-3 cytokine
Diclofenac induces microtubule depolymerization. ( A ) Confocal microscopy of HeLa cells stained with antibodies to <t>α-tubulin</t> (green) after incubation at 37 °C (upper panel) for 6 h or at 37 °C for 3 h followed by 4 °C for 3 h. Cells were incubated in a medium containing vehicle (0.1% dimethyl sulfoxide [DMSO]), nocodazole (100 nM), taxol (100 nM), or diclofenac (170 µM, 200 µM). Nuclei were stained with 4–6-diamidino-2-phenylindole (DAPI, blue). ( B ) Quantitative analysis of mean fluorescence intensity of α-tubulin. Data are presented as means ± SD from three independent experiments ( n = 52–60 cells). * p < 0.05; ** p < 0.01 (Student’s t -test). ( C ) Confocal microscopy of HepG2 cells stained with antibodies to α-tubulin (green) and LAMP1 (red) after incubation in a medium containing vehicle (0.1% DMSO) or diclofenac (500 µM) under fed (Dulbecco’s modified eagle medium, 10% fetal bovine serum) conditions or starved (Earle’s balanced salt solution) conditions for 8 h. Nuclei were stained with DAPI (blue). Representative images are shown. ( n = 14–18 cell). Areas enclosed by the white boxes are shown at higher magnification. Yellow boxes indicate the edges of the plasma membrane. Three independent experiments were performed. Scale bar, 10 µm; scale bar in magnification; 2 µm. ( D ) In vitro <t>tubulin</t> <t>polymerization.</t> Polymerization activity was monitored in the presence of DMSO (0.01%, vehicle), taxol (10 μM), nocodazole (10 μM), or diclofenac (0.17 mM and 1.7 mM) for 30 min at 37 °C as the increase in A 340 nm. Two independent experiments were performed.
Il 3 Cytokine, supplied by Immunex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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substrate p nitrophenyl α d maltoside  (Gold Biotechnology Inc)
90
Gold Biotechnology Inc substrate p nitrophenyl α d maltoside
Diclofenac induces microtubule depolymerization. ( A ) Confocal microscopy of HeLa cells stained with antibodies to <t>α-tubulin</t> (green) after incubation at 37 °C (upper panel) for 6 h or at 37 °C for 3 h followed by 4 °C for 3 h. Cells were incubated in a medium containing vehicle (0.1% dimethyl sulfoxide [DMSO]), nocodazole (100 nM), taxol (100 nM), or diclofenac (170 µM, 200 µM). Nuclei were stained with 4–6-diamidino-2-phenylindole (DAPI, blue). ( B ) Quantitative analysis of mean fluorescence intensity of α-tubulin. Data are presented as means ± SD from three independent experiments ( n = 52–60 cells). * p < 0.05; ** p < 0.01 (Student’s t -test). ( C ) Confocal microscopy of HepG2 cells stained with antibodies to α-tubulin (green) and LAMP1 (red) after incubation in a medium containing vehicle (0.1% DMSO) or diclofenac (500 µM) under fed (Dulbecco’s modified eagle medium, 10% fetal bovine serum) conditions or starved (Earle’s balanced salt solution) conditions for 8 h. Nuclei were stained with DAPI (blue). Representative images are shown. ( n = 14–18 cell). Areas enclosed by the white boxes are shown at higher magnification. Yellow boxes indicate the edges of the plasma membrane. Three independent experiments were performed. Scale bar, 10 µm; scale bar in magnification; 2 µm. ( D ) In vitro <t>tubulin</t> <t>polymerization.</t> Polymerization activity was monitored in the presence of DMSO (0.01%, vehicle), taxol (10 μM), nocodazole (10 μM), or diclofenac (0.17 mM and 1.7 mM) for 30 min at 37 °C as the increase in A 340 nm. Two independent experiments were performed.
Substrate P Nitrophenyl α D Maltoside, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glucoamylase activity assay  (Kikkoman Corporation)
90
Kikkoman Corporation glucoamylase activity assay
Diclofenac induces microtubule depolymerization. ( A ) Confocal microscopy of HeLa cells stained with antibodies to <t>α-tubulin</t> (green) after incubation at 37 °C (upper panel) for 6 h or at 37 °C for 3 h followed by 4 °C for 3 h. Cells were incubated in a medium containing vehicle (0.1% dimethyl sulfoxide [DMSO]), nocodazole (100 nM), taxol (100 nM), or diclofenac (170 µM, 200 µM). Nuclei were stained with 4–6-diamidino-2-phenylindole (DAPI, blue). ( B ) Quantitative analysis of mean fluorescence intensity of α-tubulin. Data are presented as means ± SD from three independent experiments ( n = 52–60 cells). * p < 0.05; ** p < 0.01 (Student’s t -test). ( C ) Confocal microscopy of HepG2 cells stained with antibodies to α-tubulin (green) and LAMP1 (red) after incubation in a medium containing vehicle (0.1% DMSO) or diclofenac (500 µM) under fed (Dulbecco’s modified eagle medium, 10% fetal bovine serum) conditions or starved (Earle’s balanced salt solution) conditions for 8 h. Nuclei were stained with DAPI (blue). Representative images are shown. ( n = 14–18 cell). Areas enclosed by the white boxes are shown at higher magnification. Yellow boxes indicate the edges of the plasma membrane. Three independent experiments were performed. Scale bar, 10 µm; scale bar in magnification; 2 µm. ( D ) In vitro <t>tubulin</t> <t>polymerization.</t> Polymerization activity was monitored in the presence of DMSO (0.01%, vehicle), taxol (10 μM), nocodazole (10 μM), or diclofenac (0.17 mM and 1.7 mM) for 30 min at 37 °C as the increase in A 340 nm. Two independent experiments were performed.
Glucoamylase Activity Assay, supplied by Kikkoman Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nourseothricin  (WERNER BioAgents)
90
WERNER BioAgents nourseothricin
Diclofenac induces microtubule depolymerization. ( A ) Confocal microscopy of HeLa cells stained with antibodies to <t>α-tubulin</t> (green) after incubation at 37 °C (upper panel) for 6 h or at 37 °C for 3 h followed by 4 °C for 3 h. Cells were incubated in a medium containing vehicle (0.1% dimethyl sulfoxide [DMSO]), nocodazole (100 nM), taxol (100 nM), or diclofenac (170 µM, 200 µM). Nuclei were stained with 4–6-diamidino-2-phenylindole (DAPI, blue). ( B ) Quantitative analysis of mean fluorescence intensity of α-tubulin. Data are presented as means ± SD from three independent experiments ( n = 52–60 cells). * p < 0.05; ** p < 0.01 (Student’s t -test). ( C ) Confocal microscopy of HepG2 cells stained with antibodies to α-tubulin (green) and LAMP1 (red) after incubation in a medium containing vehicle (0.1% DMSO) or diclofenac (500 µM) under fed (Dulbecco’s modified eagle medium, 10% fetal bovine serum) conditions or starved (Earle’s balanced salt solution) conditions for 8 h. Nuclei were stained with DAPI (blue). Representative images are shown. ( n = 14–18 cell). Areas enclosed by the white boxes are shown at higher magnification. Yellow boxes indicate the edges of the plasma membrane. Three independent experiments were performed. Scale bar, 10 µm; scale bar in magnification; 2 µm. ( D ) In vitro <t>tubulin</t> <t>polymerization.</t> Polymerization activity was monitored in the presence of DMSO (0.01%, vehicle), taxol (10 μM), nocodazole (10 μM), or diclofenac (0.17 mM and 1.7 mM) for 30 min at 37 °C as the increase in A 340 nm. Two independent experiments were performed.
Nourseothricin, supplied by WERNER BioAgents, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gsh assay kit bioxytech gr-340  (OXIS International)
90
OXIS International gsh assay kit bioxytech gr-340
Diclofenac induces microtubule depolymerization. ( A ) Confocal microscopy of HeLa cells stained with antibodies to <t>α-tubulin</t> (green) after incubation at 37 °C (upper panel) for 6 h or at 37 °C for 3 h followed by 4 °C for 3 h. Cells were incubated in a medium containing vehicle (0.1% dimethyl sulfoxide [DMSO]), nocodazole (100 nM), taxol (100 nM), or diclofenac (170 µM, 200 µM). Nuclei were stained with 4–6-diamidino-2-phenylindole (DAPI, blue). ( B ) Quantitative analysis of mean fluorescence intensity of α-tubulin. Data are presented as means ± SD from three independent experiments ( n = 52–60 cells). * p < 0.05; ** p < 0.01 (Student’s t -test). ( C ) Confocal microscopy of HepG2 cells stained with antibodies to α-tubulin (green) and LAMP1 (red) after incubation in a medium containing vehicle (0.1% DMSO) or diclofenac (500 µM) under fed (Dulbecco’s modified eagle medium, 10% fetal bovine serum) conditions or starved (Earle’s balanced salt solution) conditions for 8 h. Nuclei were stained with DAPI (blue). Representative images are shown. ( n = 14–18 cell). Areas enclosed by the white boxes are shown at higher magnification. Yellow boxes indicate the edges of the plasma membrane. Three independent experiments were performed. Scale bar, 10 µm; scale bar in magnification; 2 µm. ( D ) In vitro <t>tubulin</t> <t>polymerization.</t> Polymerization activity was monitored in the presence of DMSO (0.01%, vehicle), taxol (10 μM), nocodazole (10 μM), or diclofenac (0.17 mM and 1.7 mM) for 30 min at 37 °C as the increase in A 340 nm. Two independent experiments were performed.
Gsh Assay Kit Bioxytech Gr 340, supplied by OXIS International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bioxytechgr-340 kits  (OXIS International)
90
OXIS International bioxytechgr-340 kits
Diclofenac induces microtubule depolymerization. ( A ) Confocal microscopy of HeLa cells stained with antibodies to <t>α-tubulin</t> (green) after incubation at 37 °C (upper panel) for 6 h or at 37 °C for 3 h followed by 4 °C for 3 h. Cells were incubated in a medium containing vehicle (0.1% dimethyl sulfoxide [DMSO]), nocodazole (100 nM), taxol (100 nM), or diclofenac (170 µM, 200 µM). Nuclei were stained with 4–6-diamidino-2-phenylindole (DAPI, blue). ( B ) Quantitative analysis of mean fluorescence intensity of α-tubulin. Data are presented as means ± SD from three independent experiments ( n = 52–60 cells). * p < 0.05; ** p < 0.01 (Student’s t -test). ( C ) Confocal microscopy of HepG2 cells stained with antibodies to α-tubulin (green) and LAMP1 (red) after incubation in a medium containing vehicle (0.1% DMSO) or diclofenac (500 µM) under fed (Dulbecco’s modified eagle medium, 10% fetal bovine serum) conditions or starved (Earle’s balanced salt solution) conditions for 8 h. Nuclei were stained with DAPI (blue). Representative images are shown. ( n = 14–18 cell). Areas enclosed by the white boxes are shown at higher magnification. Yellow boxes indicate the edges of the plasma membrane. Three independent experiments were performed. Scale bar, 10 µm; scale bar in magnification; 2 µm. ( D ) In vitro <t>tubulin</t> <t>polymerization.</t> Polymerization activity was monitored in the presence of DMSO (0.01%, vehicle), taxol (10 μM), nocodazole (10 μM), or diclofenac (0.17 mM and 1.7 mM) for 30 min at 37 °C as the increase in A 340 nm. Two independent experiments were performed.
Bioxytechgr 340 Kits, supplied by OXIS International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Diclofenac induces microtubule depolymerization. ( A ) Confocal microscopy of HeLa cells stained with antibodies to α-tubulin (green) after incubation at 37 °C (upper panel) for 6 h or at 37 °C for 3 h followed by 4 °C for 3 h. Cells were incubated in a medium containing vehicle (0.1% dimethyl sulfoxide [DMSO]), nocodazole (100 nM), taxol (100 nM), or diclofenac (170 µM, 200 µM). Nuclei were stained with 4–6-diamidino-2-phenylindole (DAPI, blue). ( B ) Quantitative analysis of mean fluorescence intensity of α-tubulin. Data are presented as means ± SD from three independent experiments ( n = 52–60 cells). * p < 0.05; ** p < 0.01 (Student’s t -test). ( C ) Confocal microscopy of HepG2 cells stained with antibodies to α-tubulin (green) and LAMP1 (red) after incubation in a medium containing vehicle (0.1% DMSO) or diclofenac (500 µM) under fed (Dulbecco’s modified eagle medium, 10% fetal bovine serum) conditions or starved (Earle’s balanced salt solution) conditions for 8 h. Nuclei were stained with DAPI (blue). Representative images are shown. ( n = 14–18 cell). Areas enclosed by the white boxes are shown at higher magnification. Yellow boxes indicate the edges of the plasma membrane. Three independent experiments were performed. Scale bar, 10 µm; scale bar in magnification; 2 µm. ( D ) In vitro tubulin polymerization. Polymerization activity was monitored in the presence of DMSO (0.01%, vehicle), taxol (10 μM), nocodazole (10 μM), or diclofenac (0.17 mM and 1.7 mM) for 30 min at 37 °C as the increase in A 340 nm. Two independent experiments were performed.

Journal: Antioxidants

Article Title: Diclofenac: A Nonsteroidal Anti-Inflammatory Drug Inducing Cancer Cell Death by Inhibiting Microtubule Polymerization and Autophagy Flux

doi: 10.3390/antiox11051009

Figure Lengend Snippet: Diclofenac induces microtubule depolymerization. ( A ) Confocal microscopy of HeLa cells stained with antibodies to α-tubulin (green) after incubation at 37 °C (upper panel) for 6 h or at 37 °C for 3 h followed by 4 °C for 3 h. Cells were incubated in a medium containing vehicle (0.1% dimethyl sulfoxide [DMSO]), nocodazole (100 nM), taxol (100 nM), or diclofenac (170 µM, 200 µM). Nuclei were stained with 4–6-diamidino-2-phenylindole (DAPI, blue). ( B ) Quantitative analysis of mean fluorescence intensity of α-tubulin. Data are presented as means ± SD from three independent experiments ( n = 52–60 cells). * p < 0.05; ** p < 0.01 (Student’s t -test). ( C ) Confocal microscopy of HepG2 cells stained with antibodies to α-tubulin (green) and LAMP1 (red) after incubation in a medium containing vehicle (0.1% DMSO) or diclofenac (500 µM) under fed (Dulbecco’s modified eagle medium, 10% fetal bovine serum) conditions or starved (Earle’s balanced salt solution) conditions for 8 h. Nuclei were stained with DAPI (blue). Representative images are shown. ( n = 14–18 cell). Areas enclosed by the white boxes are shown at higher magnification. Yellow boxes indicate the edges of the plasma membrane. Three independent experiments were performed. Scale bar, 10 µm; scale bar in magnification; 2 µm. ( D ) In vitro tubulin polymerization. Polymerization activity was monitored in the presence of DMSO (0.01%, vehicle), taxol (10 μM), nocodazole (10 μM), or diclofenac (0.17 mM and 1.7 mM) for 30 min at 37 °C as the increase in A 340 nm. Two independent experiments were performed.

Article Snippet: The absorbance at 340 nm was measured to monitor tubulin polymerization using SpectraMax M Series Multi-Mode Microplate Readers (Molecular Devices, San Jose, CA, USA).

Techniques: Confocal Microscopy, Staining, Incubation, Fluorescence, Modification, In Vitro, Activity Assay